Ciona intestinalis early embryonic development ~ from egg to larva ~

Normal View (=> 3D reconstructed images)

Zygote period (0-0.9hr)  at 18 degrees C
Image
(Description)
Link Stage Time after
fertilization
Developmental
stage
Percentage
until hatch
Description Tail
length (µ m)
Ratio
tail/head
1 0.4hr 1cell (fertilized egg ) 2% Zygote, fertilized egg - -

Cleavage period (0.9-4.5hr)
Image
(Description)
Link Stage Time after
fertilization
Developmental
stage
Percentage
until hatch
Description Tail
length (µ m)
Ratio
tail/head
2 0.9hr 2cell 6% Two cell-stage embryo - -
3 1.45hr 4cell 8% Four cell-stage embryo - -
4 1.9hr 8cell 11% Eight cell-stage embryo - -
5a 2.35hr early 16cell 13% Early sixteen-cell stage embryo - -
5b 2.65hr late 16cell 15% Late sixteen-cell stage embryo - -
6a 3hr early 32 cell 17% Early thirty two-cell stage embryo - -
6b 3.2hr late 32cell 18% Late thirty two-cell stage embryo - -
7 3.35hr 44 cell 19% Fourty four-cell stage embryo. The vegetal side of the embryo is very round.
8 4hr 64cell 23% Sixty four-cell stage embryo. Embryo has a square shape seen form the top, with bulging B7.4 cells. - -
9 4.2hr 76 cell 24% Seventy six cell stage embryo. The vegetal side of the embryo is very flat - -

Gastrula Period (4.5-6.3hr)
Image
(Description)
Link Stage Time after
fertilization
Developmental
stage
Percentage
until hatch
Description Tail
length (µ m)
Ratio
tail/head
10 4.5hr 112cell 26% Gastrulation starts with the apical constriction of A7.1 blastomeres. - -
11 4.9hr early gastrula 28% The ntochord has invaginated. The vegetal side of the embryo has a horseshoe shape. - -
12 5.65hr mid gastrula 32% Six-row neural plate stage. The blastopore is still central and open. - -
13 5.9hr late gastrula 34% The blastopore is in posterior position and nearly closed. The embryo elongates anteriorly. The neural plate has more than 6 rows and the A-line neural rows (I and II) start to curve (neurulation begin). The large b6.5 progeny are coming together at the midline. - -

Neurula Period (6.3-8.5hr)
Image
(Description)
Link Stage Time after
fertilization
Developmental
stage
Percentage
until hatch
Description Tail
length (µ m)
Ratio
tail/head
14 6.35hr early neurula 36% A-line neural plate forms a gutter lined by b6.5 descendants. The embryo has a diamond shape. The gutter is not closed. - -
15 6.8hr mid neurula 39% The neural tube has formed on most of its length. The embryo has an oval shape. The a-line neural plate also forms a gutter. 83.0 1.0
16 7.4hr late neurula 42% The neural tube starts to form in the posterior territories. The embryo elongates. 84.2 1.0

Tailbud Period (8.5-17.5hr)
Image
(Description)
Link Stage Time after
fertilization
Developmental
stage
Percentage
until hatch
Description Tail
length (µ m)
Ratio
tail/head
17 8.45hr initial tailbud I 48% First indication of a separation between tail and trunk territories. The tail is not bent and has the same length as the trunk. Any notochord cells not finished intercalation. 87.8 1.0
18 8.8hr initial tailbud II 50% The tail is clearly separated from the trunk. Tail and trunk have same length. Neuropore still open, a-line neurulation. 111.9 1.1
19 9.3hr early tailbud I 53% The tail bends about 40°and is slightly longer than the trunk. A few anterior most notochord cells begin to intercalate and linear. 120.3 1.2
20 9.5hr early tailbud II 54% Neuropore closed, tail bent by 60°, neurulation complete. 146.7 1.3
21 10.0hr mid tailbud I 57% Tail 1 1/2 times longer than trunk and curve ventrally (90°). Intercalation of notochord cells just finished. 180.7 1.6
22 10.9hr mid tailbud II 62% The body adopts a half circle shape. Tail twice as long as trunk. 221.2 1.9
23 11.9hr late tailbud I 68% Initiation of the pigmentation of the otolith. Tail strongly curved with tip close to the anterior end of the trunk. 255.1 2.1
24 13.5hr late tailbud II 77% Notochord vacuolation begins, palps start to be visible at the front end of the embryo. Tail straightens. 442.6 3.4
25 15.9hr late tailbud III 91% Ocellus melanization. All notochord cells have vacuoles. Tail bent dorsally. 558.6 3.9

Larva Period (17.5hr-)
Image
(Description)
Link Stage Time after
fertilization
Developmental
stage
Percentage
until hatch
Description Tail
length (µ m)
Ratio
tail/head
26 17.5hr hatching larva 100% Hatching. head adopts an elongated rectangular shape. 661.6 4.2