Fourty four-cell stage embryo. The vegetal side of the embryo is very round.
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-
8
4hr
64cell
23%
Sixty four-cell stage embryo. Embryo has a square shape seen form the top, with bulging B7.4 cells.
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-
9
4.2hr
76 cell
24%
Seventy six cell stage embryo. The vegetal side of the embryo is very flat
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Gastrula Period (4.5-6.3hr)
Image (Description)
Link
Stage
Time after fertilization
Developmental stage
Percentage until hatch
Description
Tail length (µ m)
Ratio tail/head
10
4.5hr
112cell
26%
Gastrulation starts with the apical constriction of A7.1 blastomeres.
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-
4.6hr
27%
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-
11
4.9hr
early gastrula
28%
The ntochord has invaginated. The vegetal side of the embryo has a horseshoe shape.
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-
12
5.65hr
mid gastrula
32%
Six-row neural plate stage. The blastopore is still central and open.
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-
13
5.9hr
late gastrula
34%
The blastopore is in posterior position and nearly closed. The embryo elongates anteriorly. The neural plate has more than 6 rows and the A-line neural rows (I and II) start to curve (neurulation begin). The large b6.5 progeny are coming together at the midline.
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Neurula Period (6.3-8.5hr)
Image (Description)
Link
Stage
Time after fertilization
Developmental stage
Percentage until hatch
Description
Tail length (µ m)
Ratio tail/head
14
6.35hr
early neurula
36%
A-line neural plate forms a gutter lined by b6.5 descendants. The embryo has a diamond shape. The gutter is not closed.
-
-
15
6.8hr
mid neurula
39%
The neural tube has formed on most of its length. The embryo has an oval shape. The a-line neural plate also forms a gutter.
83.0
1.0
16
7.4hr
late neurula
42%
The neural tube starts to form in the posterior territories. The embryo elongates.
84.2
1.0
Tailbud Period (8.5-17.5hr)
Image (Description)
Link
Stage
Time after fertilization
Developmental stage
Percentage until hatch
Description
Tail length (µ m)
Ratio tail/head
17
8.45hr
initial tailbud I
48%
First indication of a separation between tail and trunk territories. The tail is not bent and has the same length as the trunk. Any notochord cells not finished intercalation.
87.8
1.0
18
8.8hr
initial tailbud II
50%
The tail is clearly separated from the trunk. Tail and trunk have same length. Neuropore still open, a-line neurulation.
111.9
1.1
19
9.3hr
early tailbud I
53%
The tail bends about 40°and is slightly longer than the trunk. A few anterior most notochord cells begin to intercalate and linear.
120.3
1.2
20
9.5hr
early tailbud II
54%
Neuropore closed, tail bent by 60°, neurulation complete.
146.7
1.3
21
10.0hr
mid tailbud I
57%
Tail 1 1/2 times longer than trunk and curve ventrally (90°). Intercalation of notochord cells just finished.
180.7
1.6
22
10.9hr
mid tailbud II
62%
The body adopts a half circle shape. Tail twice as long as trunk.
221.2
1.9
23
11.9hr
late tailbud I
68%
Initiation of the pigmentation of the otolith. Tail strongly curved with tip close to the anterior end of the trunk.
255.1
2.1
12hr
69%
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-
24
13.5hr
late tailbud II
77%
Notochord vacuolation begins, palps start to be visible at the front end of the embryo. Tail straightens.
442.6
3.4
25
15.9hr
late tailbud III
91%
Ocellus melanization. All notochord cells have vacuoles. Tail bent dorsally.
558.6
3.9
16.5hr
94%
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-
17.0hr
97%
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Larva Period (17.5hr-)
Image (Description)
Link
Stage
Time after fertilization
Developmental stage
Percentage until hatch
Description
Tail length (µ m)
Ratio tail/head
26
17.5hr
hatching larva
100%
Hatching. head adopts an elongated rectangular shape.